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ICCBS > Dr. Panjwani Center for Molecular Medicine and Drug ResearchAcademics > Pre-viva Examination
Pre-viva Examination
Pre-viva Examination
Course Contents for Ph.D.

Pre-viva (written) Examination Molecular MedicIne
Dr. Panjwani Center for Molecular Medicine and Drug Research (ICCBS)


Biochemistry (20%)

1.    Introduction to

§  Amino Acids, Peptides, and Proteins

§  Nucleic Acids

§  Enzymes

§  Carbohydrates

§  Lipids

2.   Protein structure and function

3.   Enzyme Kinetics, Mechanism of Enzyme Function and Regulation

4.   Metabolic Pathways

§  Glycolysis, Gluconeogenesis, and the Pentose Phosphate Pathway,

§  The Citric Acid Cycle,

§  Fatty Acid Oxidation (β-oxidation)

§  Urea Cycle

§  Oxidative Phosphorylation

5.   Biochemical Calculations

6.   Techniques in Biochemistry

§  Chromatography

§  Electrophoresis

§  Spectroscopy

 

Molecular Biology (10%)

1.    Genes and Chromosomes

§  Chromosomal Elements

§  DNA Supercoiling

§  The Structure of Chromosomes


2.   DNA Metabolism

§  DNA Replication

§  DNA Repair

§  DNA Recombination

3.   RNA Metabolism

§  DNA-Dependent Synthesis of RNA

§  RNA Processing

§  RNA-Dependent Synthesis of RNA and DNA

4.   Protein Metabolism

§  The Genetic Code

§  Protein Synthesis

§  Protein Targeting and Degradation

5.   Techniques in Molecular Biology

§  PCR

§  Electrophoresis

§  DNA Sequencing

 

Genetics (10%)

1.    Mendelian inheritance

§  Single and Multiple genes (ABO blood group)

§  Incomplete dominance

§  Sex linked inheritance (hemophilia, Duchenne musculardystrophy)

§  X-chromosome inactivation.

2.   Chromatin structure and Chromosomes

§  Features of eukaryotic chromosome (Centromere, Secondary constrictions, Telomeres, Euchromatic region, Heterochromatic region).

§  Chromosome number and speciation

§  Packaging of genetic material in phages, bacteria and eukaryotes

§  Chromatin with various levels of organization

§  Histones (nucleosomes and their modifications)

3.   Karyotypes, Chromosomal Abberations

§  Karyotypes of normal and diseased individuals,

§  Chromosomal Abberations (deletion, duplication, inversions and translocation)

4.   Introns and Exons, Single copy and Repetitive DNA, Denaturation and
re-naturation kinetics, similarity and complementarity between sequences

5.   Transposable elements, Evidences for mobile elements

6.   Gene regulation and expression in prokaryotes and eukaryotes

§  DNA methylation

§  Imprinted genes

§  Histone modifications

§  Overlapping genes

§  beta globin and ovalbumin genes

§  Augenous control (tubulin)

§  Anti-sense RNA (lin 14 C. elegans)

§  Response elements (heat shock, glucocorticoid and serum)

§  Transcription factors

7.   Genes controlling the development of Drosophila (early development head / tail polarity), Homeotic genes.

 

Cell Biology (20%)

 

n  Visualizing cells (Chapter 09)

n  Membrane structure (Chapter 10)

n  Membrane transport of small molecules and the electrical properties of membranes (Chapter 11)

n  Mechanisms of cell communication (Chapter 15)

n  The cytoskeleton (Chapter 16)

n  The cell cycle (Chapter 17)

Reference

Molecular Biology of the Cell, 5th Edition

Alberts, Johnson, Lewis, Raff, Roberts and Walter 2008

ISBN: 978-0-8153-4105-5

Pharmacology (20%)

1.       Pharmacology: Branches, routes of drug administration and dosage forms

2.      Pharmacokinetics

3.      Pharmacodynamics and second messenger system

4.      Signal transduction

5.      Antifungal Drugs

6.      Antiviral Drugs

7.      Antiprotozoal Drugs

8.      Antibacterial Drugs

9.      Anticancer Drugs

 

Immunology (20%)

1.    Major characteristics/functions of the immune system

§  Specificity

§  Immunologic memory

§  Discrimination between self and non self

2.   Cells and organs constituting the immune system

§  Primary lymphoid organs (Thymus and bone marrow)

§  Secondary lymphoid tissue (spleen and lymph nodes)

3.   The immunocompetent cells

§  Origin, cell surface antigens, populations and subpopulations of Lymphocytes and Phagocytes (T cells, B Cells, Natural Killer (NK) Cells, Macrophages, Dendritic Cells, Neutrophils.)

4.   Types of Immune Mechanisms

§  Non specific host resistance (Innate immunity)

§  Chemical and cellular mediators of innate immunity

§  Mechanisms of specific host defense (Adaptive Immunity)

§  Humoral Immune response / antibody production and structure

§  Cells involved in the cellular sequence of events and functions of antibody

§  Structure, types and functions of antibodies

5.   The major histocompatibility complex and cell mediated immune response

§  Major histocompatibility complex (MHC) molecules

§  Role of the MHC in the immune response

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