Molecular Bank

PCMD Molecular Bank: A National Resource for Drug Discovery and Development:


Compounds libraries are being develop all around the world to store millions of potential drug candidates. Proper management of the Compound collection is the foundation of any quality Compound screening program.

The first Molecular Bank of Pakistan, established at the Dr. Panjwani Center for Compound Medicine and Drug Research, offers state-of–the–art technology and expertise in the field of screening of chemical diversity for drug discovery and development. PCMD’s Molecular Bank serves as an open platform for chemists and biologists with the aim to support research on diverse small Compound libraries for their potential as leads, relevant to drug discovery.

Molecular Bank facilitate to organize the Compounds library into a 96-well plate format for High and Medium Throughput Screenings (HTS) against well-defined biological targets, such as enzymes. 
 
Molecular Bank Stock:   

Compounds collection is contributed by the isolation and synthetic research groups of the International Center for Chemical and Biological Sciences. More than 14,500 structurally diverse synthetic and natural Compounds of different classes are currently available in the PCMD Molecular Bank for bioassay screening, drug discovery and design, as well as for other chemical and biological research.Compounds Preservation and Library Design:

Compounds are preserved in the following formats, Dry form: 5 mg and > 5 mg
Compounds preserved in screw capped glass vials with specific barcodes at -20 ˚C
Solution form:10 mM and 20 mM in DMSO

Compounds preserved in 96-well plate, sealed with adhesive foil with specific barcode at -20 ˚C. Each mother plate have 4 daughter plates for back-up, and for screening purposes.
 
Bioassays:   

The PCMD Molecular Bank, an open interdisciplinary platform, offers a broad spectrum of bioassays relevant to study of preventing diseases such as cancer, Alzheimer’s and Parkinson’s diseases, diabetes, inflammation, arthritis, arthrosclerosis, malaria, infections, resistant microbial infections, oxidative stress, rheumatoid arthritis, tropical diseases, etc. Various kinds of assays are set-up with liquid handling in 96-well plate format in order to minimize the utilization of Compounds and reagents.
 
 
Following biological assays are routinely conducted by the Molecular Bank of PCMD.
 
(a) Enzyme Inhibition Studies by Biochemical Assays (b) Antioxidant Studies (c) Bench-top Bioassays
Phosphodiesterase Inhibitio DPPH Radical Scavenging Assay Antibacterial Activity
Urease Inhibition ABTS Radical Scavenging Assay Antifungal Activity
Thymidine Phosphorylase Inhibition Superoxide Anion Scavenging Assay Insecticidal Activity
Acetylecholinesterase Inhibition Nitric Oxide Scavenging Assay Phytotoxic Activity
Butyrylcholinesterase Inhibition Xanthine Oxidase Inhibition Assay Brine-Shrimp Lethality Assay
∞- Glucosidase Inhibition Fe Chelating Assay  

∞- Chymotrypsin Inhibition
β-Glucuronidase Inhibition
Carbonic Anhydrase Inhibition
Tyrosinase Inhibition
Lypoxygenase Inhibition

(a) Antiglycation (Proteins) Studies
(b) Immunomodulatory/ Cytotoxicity Assays
(c) Antileishmanial Assays against Leishmania major
(d) Cytotoxicity Assays against normal cell lines
(e) Anticancer Assays against a range of cancer cell lines


Liquidator, manually operated high performance 96 channel pipetting device, Volume range: 1µl to 200 µl, Manual; NO software programming is required.
 
Descripition of the database and data files:   

It is prudent for a large organization to have a chemically intelligent database and compatible management of the Compounds for further research and development. In PCMD Molecular Bank, Compounds are managed with barcodes and relational database. Chemfinder database is used to maintain chemical, structural and biological information for ready reference. Molecular Bank Database is home-based, which is only accessible to the ICCBS researchers through institutional network.
Chemfinder for excel creates text data in spreadsheets.
Chemfinder for database creates SDF. SDF is the structure data file which shows the structural information along with chemical and biological data with respect to the structure identification code (structure ID). 
 
Standards and IP Protection:   

The bioassay services are generally rendered free of cost with mutual agreement. In case of any promising active Compound discovery, collaboration will be established between the chemists and bioassay screening experts for joint patent and publication. As per PCMD Molecular Bank standards, the Compounds should have a purity of 99% (Compounds are checked for purity, quantity, and authenticity). All chemical and biological information must be provided by the sample provider.
 
Compounds Submission:   

If you are interested in biological analysis of your Compound, we invite you to send us atleast 5-10 mg of test samples in pre-weighed Eppendroff tubes or vials, along with relevant information; such as structure, Compound weight, solubility, stability, potential hazards and relevant reference. Before submitting the samples, kindly make sure that your Compound is free from all types of solvents and other chemical impurities.
 
Download sample submission forms:   

Sample Submission Form for Collaborators (Outside Pakistan)
Sample Submission Form / H.E.C. Analysis
 
Contact Us:   

Sadia Siddiq
Molecular Bank Manager
Dr. Panjwani Center for Compound Medicine and Drug Research
International Center for Chemical and Biological Sciences
 
Phone: +922134819012
Fax: 34819018-9
Mail:  sadia@iccs.edu, sadiabio@yahoo.com